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Part:BBa_K2088673:Design

Designed by: Ping Xiaofei   Group: iGEM16_NAU-CHINA   (2016-10-09)


copA+RiboJ+GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 914


Design Notes

We want to use RiboJ to block the interaction between copA promoter and RBS(B0030) so we did not add any other base between them.


Source

We get the gene sequence from https://parts.igem.org/Part:BBa_K1555000 and https://parts.igem.org/Part:BBa_K1679038. We synthetised this part.

References